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polyvinyl difluoride pvdf membranes  (Bio-Rad)
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Polyvinyl Difluoride Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinyl difluoride pvdf membrane  (Bio-Rad)
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Bio-Rad polyvinyl difluoride pvdf membrane
Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyvinyl difluoride pvdf membrane/product/Bio-Rad
Average 96 stars, based on 1 article reviews
polyvinyl difluoride pvdf membrane - by Bioz Stars, 2026-03
96/100 stars
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polyvinyl difluoride membrane  (Bio-Rad)
96
Bio-Rad polyvinyl difluoride membrane
Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinyl difluoride (pvdf) membrane  (Millipore)
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Millipore polyvinyl difluoride (pvdf) membrane
Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride (Pvdf) Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinyl difluoride membranes  (Millipore)
90
Millipore polyvinyl difluoride membranes
Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyvinyl difluoride membranes/product/Millipore
Average 90 stars, based on 1 article reviews
polyvinyl difluoride membranes - by Bioz Stars, 2026-03
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polyvinyl difluoride membrane  (Millipore)
90
Millipore polyvinyl difluoride membrane
Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyvinyl difluoride membrane/product/Millipore
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polyvinyl difluoride membrane  (Merck KGaA)
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Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyvinyl difluoride (pvdf) membranes  (Merck KGaA)
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Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. <t>PVDF</t> membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.
Polyvinyl Difluoride (Pvdf) Membranes, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. PVDF membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.

Journal: Cells

Article Title: The Capability to Undergo ACSL4-Mediated Ferroptosis Is Acquired During Brown-like Adipogenesis and Affected by Hypoxia

doi: 10.3390/cells14161247

Figure Lengend Snippet: Effects of hypoxia in SGBS organoids. ( a – c ) Western blot analysis of normoxic (N) and hypoxic (H) SGBS organoids. n = 3; one-sample t test. ( d ) Analysis of protein oxidation by OxyBlot. A representative result of n = 3 independent experiments is shown. The DNPH substrate was added (+) or omitted (−) to confirm signal specificity. PVDF membrane was counterstained with EZBlue. ( e ) SA β-Gal staining of SGBS organoids. Grid: 100 µm. ( f ) Cytokine array. Cell culture supernatants of normoxic and hypoxic SGBS organoids of n = 3 experiments were pooled and subjected to cytokine measurement. TAT-Cyclo was used to inhibit HIF-1α. Top ten hypoxia-induced cytokines are indicated: (1) IL-6R, (2) CCL3, (3) TPO, (4) CCL27, (5) CCL16, (6) BTC, (7) CXCL8, (8) Adiponectin, (9) TIMP-2 and (10) TIMP-1. (+) Array positive control. Spearman correlation was used to analyze various parameters in clinical samples. p values ≤ 0.05 were considered to be significant as indicated: * = p < 0.05.

Article Snippet: Protein samples (5–10 μg) were separated by polyacrylamide gel electrophoresis and blotted onto a Polyvinyl Difluoride (PVDF) membrane with the semi-dry TransBlot Turbo Transfer System (BioRad).

Techniques: Western Blot, Membrane, Staining, Cell Culture, Positive Control